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. 2005 Sep 15;6(1):105. doi: 10.1186/1465-9921-6-105

Figure 1.

Figure 1

Dose-dependent increase in IL-8 expression by HCV core protein. A, NHLF were subjected to mock treatment or treatment with β-galactosidase or (β-gal) or β-galactosidase-HCV core protein (Core) at the indicated concentrations, incubated for 24 h, and supernatants assayed for IL-8 by ELISA as described in Materials and Methods. Experiments were done in triplicate. B, NHLF were subjected to mock treatment or treatment with β-galactosidase (β-gal) or β-galactosidase-HCV core protein (Core) at the indicated concentrations and incubated for 24 h. Lysates were harvested, RNA isolated and reverse transcribed, and IL-8 detected using IL-8 specific primers or HPRT as a control for RNA loading as described in Materials and Methods.