Table 1.

Kinetics of hSGLT1 in different systems

	Xenopus oocytes			Proteoliposomes
	hSGLT1*	hSGLT1-FLAG	hSGLT1-FLAG-GFP	hSGLT1ΔN-GFP
K, mM†	0.41  ± 0.03	0.33  ± 0.02	0.38  ± 0.1	0.35  ± 0.05
Turnover number, s−1‡	50	48	10	4

^*The K and the turnover number for hSGLT1 wild type and the cassette version of hSGLT1 (see Experimental Procedures) are indistinguishable. 

^† The K in oocytes was determined at −50 mV. Data are obtained from representative measurements in single oocytes (repeated in triplicate with oocytes from different batches) and the error described the SE of the fit. The K for the transporter reconstituted into proteoliposomes represents the mean of at least three independent measurements with errors indicating the SEM. 

^‡ The catalytic turnover number for the reconstituted transporter in proteoliposomes was determined by the ratio of V_max:number of transporters, whereas that for the transporter in oocytes was based on electrophysiological experiments using the maximum current (I_max):maximum charge (Q_max) ratio (24).