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Inhibitors of MAPK cascades abolish synergistic activation of the mPer1 promoter by forskolin and EGF or TPA. Transfection, treatments, and luciferase analysis were conducted as in Fig. 3B, except that PD 98059 (30 μM) or SB 203580 (10 μM) or the vehicle was applied to the cells 1 h before the inducing treatments.
