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Comparison of Co-Cyt c and Har-Co-Cyt c refolding kinetics measured by CD spectroscopy (228 nm). (a) Unmodified horse Co-Cyt c (red), horse Har-Co Cyt c (blue); (b) unmodified tuna Co-Cyt c (magenta), tuna Har-Co-Cyt c (green). Folding was initiated by manual dilution of a 4.0 M GuHCl solution to give a final denaturant concentration of 2.0 M at 25°C. The horse and tuna proteins were incubated in unfolding solutions for 10 min and 2 days, respectively.
