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. Author manuscript; available in PMC: 2025 Sep 15.
Published in final edited form as: J Vis Exp. 2024 Mar 29;(205):10.3791/66584. doi: 10.3791/66584

Materials

Name Company Catalog Number Comments
#11 Scalpel Blades World Precision Instruments 504170 For EMG electrode fabrication
#3 Scalpel Handle Fine Science Tools 10003-12 For EMG electrode fabrication
1 mL Sub-Q Syringe (100 pack) Becton Dickinson 309597 For administering injectable drugs
12-pin connector Newark 33AC2371 12-pin connector with brass fittings; for EMG electrode fabrication
18 G Needles Exel International 26419 For EMG electrode fabrication
27 G Needles Exel International 26426 For EMG electrode fabrication
3 M Transpore Surgical Tape 3M 1527-0 For taping animal's limbs out during surgery
6-0 silk sutures Henry Schein 101-2636 These sutures work well with delicate skin around the wrists
C&B Metabond Complete Kit Pearson Dental P16-0126 Dental cement to affix connector to headplate
C57BL6/J Mice Jackson Laboratories #000664 Wild type mice
Carbofib 5-CF Tweezers (2) Aven tools 18762 Carbon fiber tipped forceps, used to manipulate delicate parts of electrode (stripped or inserted sections)
Carprodyl (Carprofen) 50 mg/mL Injection Ceva Animal Health, LLC G43010B Injectable analgesic for pain management during and after surgery
Castroviejo Micro Needle Holder Fine science tools 12060-01 For suturing
Castroviejo Needle Holder (large) Fine science tools 12565-14 For inserting needle into muscle
Delicate Bone Scraper Fine science tools 10075-16 To separate skin from underlying tissue
Dietgel 76A Dietary Supplement Clear H2O 72-07-5022 For post-operative care
Dumont #5/45 Forceps Fine science tools 11251-35 To remove fascia overlying muscle
Elizabethan collar for mouse Kent Scientific Corporation EC201V-10 For post-operative care
Enrofloxacin 2.27% Covetrus #074743 Injectable antibiotic for use during and after surgery
Epoxy gel Devcon 14265 For EMG electrode fabrication
Hopkins Bulldog Clamp (4) Stoelting 10-000-481 Tissue clamps for headplate implantation
Isoflurane Solution Covetrus 11695067771 Inhalable anesthesia
Lidocaine Hydrochloride Injectable - 2% Covetrus #002468 Topical analgesic for pain management during surgery
Medical Grade Oxygen Airgas OX USP200 For administering isoflurane during surgery
MetriCide 1 Gallon Metrex 10-1400 Glutaraldehyde solution for cold- sterilization of headplate and electrodes
MetriTest Strips 1.5% Metrex 10-303 Test strips for monitoring glutaraldehyde solution (recommended)
Model 900LS Small Animal Stereotaxic Instrument Kopf Instruments 900LS Stereotax with lazy susan feature that allows platform rotation during surgery
PFA-coated 0.0055" braided stainless steel wire A-M systems 793200 For EMG electrode fabrication
Povidone-iodine prep pads Dynarex 1108 For cleaning skin
Puralube Vet Ointment Dechra 37327 Eye ointment for surgery
Sterile alcohol prep pads Dynarex 1113 For cleaning skin
Straight fine #5 forceps Fine science tools 11295-10 For curling wire after insertion
Straight fine scissors Fine science tools 14060-11 For cutting wire
Student Vannas Spring Scissors Fine science tools 91500-09 For making incisions, trimming fat and fascia, and suturing
Technik Tweezers 7B-SA (2) Aven tools 18074USA Curved blunt forceps, for general use during surgery
Triple Antibiotic Ointment Walgreens 975863 Topical antibiotic for surgery
V-1 Tabletop Laboratory Animal Anesthesia System VetEquip 901806 Contains all necessary equipment for anesthesia induction and scavenging including vaporizer, induction chamber, moveable plastic nose cone, and tubing