Fig. 10.

(a) Fluorescence intensity ratios of red (650 nm) to green (560 nm) channels from pH-dependent optical fluorescence measurements in solution (fluorometer), HeLa cells, and Drosophila melanogaster first-instar larvae treated with 10 μm probe A in buffers of varying pH. (b) Fluorescence spectra of probe A in buffer (pH 8.29) and in a 50% glycerol mixture. (c) Fluorescence spectra of probe AH+ in buffer (pH 3.50) with varying concentrations of glycerol. Error bars based on n = 3 determinations.