Fig. 11.

Fluorescence images of HeLa cells were captured following 320 minutes of treatment with different concentrations of nystatin (10, 20, and 30 μM) at pH 3.8. Green fluorescence was visualized using Alexa Fluor 405 (excitation at 405 nm), and red fluorescence was monitored using Alexa Fluor 568 (excitation at 559 nm). All images are presented with a scale bar of 50 μm. The histograms illustrate the emission intensities of channel I (blue), and channel II (red) (top), and the fluorescence intensity ratio of channel II to channel I (green) for probe A in HeLa cells (bottom), as quantified from the corresponding fluorescence images with SD bars based on n = 3.