Fig. 9.

(a) Fluorescence images of Drosophila melanogaster first-instar larvae treated with 10 μM probe A in buffers of varying pH were captured. The green channel (channel I) was used to visualize the probe’s fluorescence within the emission range of 425–525 nm, while the red channel (channel II) detected near-infrared fluorescence from 600–700 nm. Green fluorescence was observed using Alexa Fluor 405 (excitation at 405 nm), and red fluorescence was monitored using Alexa Fluor 568 (excitation at 559 nm). All images are presented with a scale bar of 50 μm. (b) The histogram displays the emission intensities of channel I (green) and channel II (red) for probe A in D. melanogaster first-instar larvae at different pH levels, as quantified from the fluorescence images. The error bars are based on experiments conducted three times.