Table 1.

Sequence of primers used to assess human sperm PRM1 and PRM2 transcript levels

Gene symbol	Primer sequence (5 $\text{'}$ -3 $\text{'}$ )
PRM1	Forward: 5 $\text{'}$ -ACCGCCAGAGACAAAGAAGT-3 $\text{'}$ Reverse: 5 $\text{'}$ -TCTACATCGCGGTCTGTACC-3 $\text{'}$
PRM2	Forward: 5 $\text{'}$ -ACCAGATCTCCCAACACCAT-3 $\text{'}$ Reverse: 5 $\text{'}$ -CAACTGCTGCCTGTACACCT-3 $\text{'}$
$\beta$ -actin	Forward: 5 $\text{'}$ -ATTGGCAATGAGCGGTTC-3 $\text{'}$ Reverse: 5 $\text{'}$ -TGAAGGTAGTTTCGTGGATG-3 $\text{'}$
PRM1: Protamine1, PRM2: Protamine2, $\beta$ -actin: Beta-actin