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. Author manuscript; available in PMC: 2025 Sep 16.
Published in final edited form as: Structure. 2025 Aug 26;33(11):1831–1843.e4. doi: 10.1016/j.str.2025.07.022

Figure 4. Secretory stimuli cause shifts in ISG spatial distribution.

Figure 4.

(A) Cartoon of the ISG maturation pathway with the ISGs color coded based on location with respect to cell membrane. Normalized EDT on the right indicates where the ISG is located (EDT: 0 –Cell membrane; EDT: 1 - furthest from the cell membrane). Different ISG subpopulations are defined based on spatial localization through the normalized EDT: Docked ISGS (EDT: 0 – 0.02), Readily Releasable Pool (RRP) (EDT: 0.02 – 0.10) and Internal ISGs or Reserve Pool (EDT: 0.1 – 0.6). (B-E) ISG LAC distribution for each 0.1 EDT zone across the cell. The last EDT zone is from 0.6 to 1.0. Each point represents the mean LAC of the ISGs for the corresponding condition in that specific EDT range. Error bars represent 95% CI. As compared to ISGs from glucose-only treated (red) cells, ISGs from: (B) Glimepiride treated cells (yellow) mostly show no significant change in ISG LAC values or trends as compared to ISGs from glucose-only treated cells (red). (C) GKA-50 treated cells (green) show a significant difference in LAC values (D) TAK-875 treated cells (purple) show a significant difference in LAC values and an upward trend in LAC going towards the membrane. (E) GIP treated cells (cyan) show no significance in LAC and Ex-4 treated cells (blue) show a significant increase in LAC. Two-way ANOVA used as a statistical test for (B-E). *p<0.05; **p<0.01: ***p<0.001; ****p<0.0001