| Chemicals, peptides, and recombinant proteins |
| Addex Bio Optimized RPMI media |
Addex Bio |
Cat#C0004-02 |
| RPMI 1640 media |
Thermo Scientific |
Cat#11875093 |
| Heat inactivated FBS |
Cytiva Lifesciences |
Cat#83007 |
| PBS |
Thermo Scientific |
Cat#10010023 |
| 1xPen/Strep |
Thermo Scientific |
Cat#15140122 |
| β-mercaptoethanol |
Thermo Scientific |
Cat#21985023 |
| Glucose |
Thermo Scientific |
Cat#A2494001 |
| NaCl |
Sigma |
Cat#S9888 |
| NaHCO3
|
Sigma |
Cat#S5761 |
| KCl |
Sigma |
Cat#746436 |
| MgCl2
|
Sigma |
Cat#M2670 |
| CaCl2.2H20 |
Sigma |
Cat#C7902 |
| BSA |
Millipore Sigma |
Cat#A9576 |
| HEPES |
Sigma |
Cat#H4034 |
| Glimepiride |
MedChemExpress |
Cat#HY-B0104 |
| GKA-50 |
MedChemExpress |
Cat#HY-15671 |
| TAK-875 |
MedChemExpress |
Cat#HY-10480 |
| GIP |
Tocris Biosciences |
Cat#2084 |
| Exendin-4 |
MedChemExpress |
Cat#HY-13443 |
| Accumax Dissociation Solution |
Innovative Cell Technologies Inc. |
Cat#AM105 |
| DNase I |
Roche Diagnostics |
Cat#5401020001 |
| Liberase TL Research Grade |
Roche Diagnostics |
Cat#10104159001 |
| Tween-20 |
Sigma-Aldrich |
Cat#P1379 |
| Triton X-100 |
Sigma-Aldrich |
Cat#93443 |
| Pierce Protease and Phosphatase Inhibitor |
Thermo Scientific |
Cat#A32961 |
| LysoTracker Red DND-99 |
Invitrogen |
Cat#L7528 |
| BODIPY 493/503 |
MedChemExpress |
Cat#HY-W090090 |
| Critical commercial assays |
| Rat Insulin ELISA kit |
Mercodia |
Cat#10-1220-01 |
| Rat/Mouse Proinsulin ELISA kit |
Mercodia |
Cat#10-1232-01 |
| Mouse Insulin ELISA Kit |
Mercodia |
Cat#10-1247-01 |
| Experimental models: Cell lines |
| INS-1E |
Merglen et al81
|
RRID:CVCL_0351 |
| Experimental models: Organisms/strains |
| STOCK Tg(Ins1-INS/sfGFP)369Arvn/J |
Zhu et al82
|
RRID: IMSR_JAX:035812 |
| Software and algorithms |
| ImageJ–Fiji1.54f |
Schindelin et al83
|
https://imagej.net/ij/
|
| GraphPad Prism 10 |
GraphPad |
https://www.graphpad.com/scientific-software/prism/
|
| Amira 2021.2 – 2023.1.1 |
Thermo Scientific |
https://thermofisher.com/amira-avizo
|
| IMOD–4.10.42 |
Kremer at al84
|
https://bio3d.colorado.edu/imod/
|
| AREC reconstruction |
Parkinson et al37
|
N/A |
| ACSeg |
Erozan et al85
|
https://biomedisa.info/gallery/
|
| Blender v4.4 |
Blender Foundation |
https://www.blender.org/
|
| Leica Application Suite X 4.1.1 |
Leica |
https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/
|
| Imaris 10.1 |
Oxford Instruments |
https://imaris.oxinst.com/
|
| SoftWoRx 7.2.2 |
Cytiva Lifesciences |
https://download.cytivalifesciences.com/cellanalysis/download_data/softWoRx/softworx_downloads.htm
|
| Organelle identification pipeline |
White et al18
|
N/A |
