Table 2.
Epigenome-wide significantly differentially methylated CpG sites (p ≤ 6.409e–08) in patients with anxiety disorders (N = 378) vs. healthy controls (N = 295).
| CpG | T-Value | P-Value | Mean of Patients - Controls (Δβ) | Nearest Gene Annotation |
|---|---|---|---|---|
| cg16581032 | 14.39 | 4.08e–41 | 0.018 (11.22%) | LINC00293 |
| cg22519184 | 11.6 | 3.17e–27 | 0.099 (28.63%) | LOC101928626 |
| cg26679879 | 8.33 | 1.06e–15 | 0.069 (23.62%) | LOC101928626 |
| cg24390820 | −8.13 | 2.16e–15 | −0.007 (−0.93%) | RIMBP2 |
| cg00689014 | −8.13 | 2.35e–15 | −0.013 (−2.2%) | GAST |
| cg02339888 | −7.98 | 6.54e–15 | −0.017 (−3.18%) | IL12RB2 |
| cg26077214 | −7.94 | 8.64e–15 | −0.021 (−3.35%) | LINC01725 |
| cg03600868 | −7.8 | 2.60e–14 | −0.019 (−2.68%) | SPTBN1 |
| cg16636182 | −7.79 | 2.70e–14 | −0.008 (−0.87%) | KDM4B |
| cg15403961 | −7.61 | 1.05e–13 | −0.023 (−3.9%) | GALNTL6 |
| cg26325497 | −7.61 | 1.06e–13 | −0.017 (−4.44%) | MIR519B |
| cg06567972 | −7.55 | 1.52e–13 | −0.01 (−1.64%) | SNRNP48 |
| cg03470837 | −7.53 | 1.72e–13 | −0.016 (−2.67%) | GABBR2 |
| cg09414262 | −7.52 | 1.83e–13 | −0.015 (−1.91%) | LINC00895 |
| cg25746764 | 7.47 | 2.76e–13 | 0.004 (1.2%) | ACBD3 |
| cg02969418 | −7.38 | 5.05e–13 | −0.014 (−2.02%) | TAF2 |
| cg11992550 | −7.33 | 7.69e–13 | −0.017 (−2.67%) | MFGE8 |
| cg23849483 | −7.31 | 8.29e–13 | −0.014 (−2.11%) | SYDE1 |
| cg01597342 | −7.26 | 1.18e–12 | −0.019 (−2.77%) | DLGAP4-AS1 |
| cg01706621 | −7.22 | 1.55e–12 | −0.017 (−2.35%) | LINC00052 |
| cg06531158 | −7.21 | 1.63e–12 | −0.001 (−0.08%) | LINC00445 |
| cg10013501 | −7.12 | 2.95e–12 | −0.004 (−0.46%) | RUNX3 |
| cg27233071 | −7.12 | 3.07e–12 | −0.026 (−4.86%) | SULT1C2P1 |
| cg04646695 | −7.04 | 5.00e–12 | −0.016 (−2.65%) | IZUMO1R |
| cg25213418 | −7.03 | 5.23e–12 | −0.014 (−2.04%) | TAOK1 |
| cg11415852 | −7.03 | 5.30e–12 | −0.012 (−1.46%) | SORBS1 |
| cg16559448 | −7.02 | 5.64e–12 | −0.019 (−3.03%) | CNOT6 |
| cg07437919 | −7.03 | 5.74e–12 | 0.001 (0.18%) | SLC45A4 |
| cg14001871 | −7.01 | 6.56e–12 | −0.006 (−0.72%) | ATP11A |
| cg10508217 | −6.97 | 8.10e–12 | −0.005 (−0.7%) | GNG12-AS1 |
| cg15609150 | −6.96 | 8.73e–12 | −0.019 (−3.3%) | MAML1 |
| cg00449068 | −6.95 | 9.57e–12 | −0.017 (−2.41%) | BEND3 |
| cg00590386 | −6.93 | 1.06e–11 | −0.007 (−1.49%) | RIC1 |
| cg00335957 | −6.93 | 1.10e–11 | −0.01 (−1.54%) | CWC27 |
| cg22565389 | −6.92 | 1.14e–11 | −0.002 (−0.33%) | CNN1 |
| cg22887498 | −6.91 | 1.17e–11 | −0.005 (−0.57%) | CCER2 |
| cg15817341 | −6.86 | 1.62e–11 | −0.015 (−1.94%) | ANXA6 |
| cg14264457 | −6.84 | 1.88e–11 | −0.007 (−0.89%) | CCDC68 |
| cg12009816 | −6.82 | 2.11e–11 | −0.014 (−1.77%) | RPH3AL |
| cg01523759 | −6.81 | 2.22e–11 | −0.003 (−0.36%) | ACSF3 |
| cg08580015 | −6.8 | 2.40e–11 | −0.001 (−0.15%) | MCPH1 |
| cg05869737 | −6.79 | 2.52e–11 | −0.015 (−2.58%) | APPBP2 |
| cg22865640 | −6.78 | 2.73e–11 | −0.006 (−1.05%) | MAFB |
| cg20324516 | −6.76 | 3.16e–11 | −0.018 (−2.37%) | LINC01250 |
| cg26529712 | −6.76 | 3.20e–11 | −0.015 (−2.79%) | LINC01838 |
| cg19763069 | −6.74 | 3.45e–11 | −0.01 (−1.38%) | LOC101928737 |
| cg26420606 | −6.74 | 3.50e–11 | −0.006 (−0.75%) | L3MBTL4 |
| cg03513682 | −6.71 | 4.16e–11 | −0.007 (−0.93%) | GSE1 |
| cg26903053 | −6.69 | 4.78e–11 | −0.003 (−0.34%) | GABRD |
| cg15229773 | −6.69 | 4.82e–11 | −0.023 (−3.27%) | PATE2 |
| cg15339796 | −6.69 | 5.01e–11 | −0.016 (−2.2%) | TRIB3 |
| cg01739401 | −6.68 | 5.31e–11 | −0.017 (−2.37%) | ATF1 |
| cg04258637 | −6.66 | 5.96e–11 | −0.026 (−4.95%) | COX6C |
| cg09754810 | −6.65 | 6.04e–11 | −0.013 (−1.75%) | ZFAND3 |
| cg20191924 | −6.64 | 6.54e–11 | −0.012 (−1.54%) | ACCS |
| cg01712359 | −6.63 | 6.93e–11 | −0.003 (−0.32%) | PIEZO1 |
| cg07320742 | −6.63 | 7.12e–11 | −0.016 (−2.28%) | TAX1BP1 |
| cg20750930 | −6.63 | 7.21e–11 | −0.003 (−0.4%) | FBRSL1 |
| cg00986191 | −6.63 | 7.28e–11 | −0.01 (−1.22%) | GRM6 |
| cg10636163 | −6.63 | 7.29e–11 | −0.018 (−2.35%) | C3orf67 |
| cg03916902 | 6.63 | 7.58e–11 | 0.004 (2.46%) | AP4S1 |
| cg11277380 | −6.62 | 7.75e–11 | −0.012 (−1.61%) | CCDC105 |
| cg21242417 | −6.61 | 7.81e–11 | −0.009 (−1.39%) | EXD3 |
| cg15610777 | −6.61 | 7.95e–11 | −0.005 (−0.6%) | CALHM3 |
| cg15604046 | −6.61 | 8.09e–11 | −0.018 (−2.97%) | TRHDE |
| cg22404741 | −6.61 | 8.41e–11 | −0.014 (−1.97%) | LINC02285 |
| cg26134615 | −6.6 | 8.69e–11 | −0.005 (−0.97%) | OR1A1 |
| cg00777895 | 6.58 | 9.89e–11 | 0.016 (4.38%) | SRP9 |
| cg17272899 | −6.57 | 1.04e–10 | −0.006 (−0.78%) | CTIF |
| cg11598427 | −6.56 | 1.11e–10 | −0.005 (−0.66%) | SMOC2 |
| cg11450546 | −6.56 | 1.13e–10 | −0.006 (−0.97%) | LYPD3 |
| cg22012693 | −6.55 | 1.15e–10 | −0.019 (−2.87%) | COL22A1 |
| cg18096895 | −6.55 | 1.16e–10 | −0.005 (−0.66%) | BATF2 |
| cg20979128 | −6.55 | 1.17e–10 | −0.005 (−0.52%) | ZBTB17 |
| cg02120071 | −6.55 | 1.17e–10 | −0.003 (−0.31%) | LOC100499194 |
| cg16311883 | −6.54 | 1.31e–10 | −0.019 (−3.46%) | RFLNA |
| cg05348295 | 6.52 | 1.37e–10 | 0.007 (8.41%) | FAM129B |
| cg22513166 | −6.51 | 1.64e–10 | −0.015 (−4.55%) | PPP2R5A |
| cg26921458 | −6.5 | 1.71e–10 | −0.007 (−0.95%) | SEPT9 |
| cg03019505 | −6.49 | 1.72e–10 | −0.014 (−2.87%) | TFIP11 |
| cg02932021 | −6.48 | 1.84e–10 | −0.01 (−1.46%) | TMC5 |
| cg02944953 | −6.47 | 1.92e–10 | −0.009 (−1.13%) | SIPA1L3 |
| cg25372841 | −6.47 | 1.93e–10 | −0.005 (−0.68%) | CYHR1 |
| cg02655549 | −6.46 | 2.08e–10 | −0.01 (−2.18%) | GPN1 |
| cg18177613 | −6.46 | 2.11e–10 | −0.009 (−1.23%) | CFAP77 |
| cg21250048 | −6.44 | 2.39e–10 | −0.021 (−3.01%) | PTEN |
| cg08314795 | −6.44 | 2.46e–10 | −0.016 (−2.15%) | LINC01728 |
| cg20680284 | −6.42 | 2.67e–10 | −0.01 (−1.59%) | LOC401324 |
| cg15695045 | −6.42 | 2.74e–10 | −0.005 (−1.09%) | PLEKHA6 |
| cg24172570 | −6.39 | 3.11e–10 | −0.013 (−1.65%) | HIBADH |
| cg10117405 | −6.4 | 3.15e–10 | 0 (−0.02%) | SRL |
| cg14224170 | −6.38 | 3.30e–10 | −0.016 (−2.33%) | SAFB2 |
| cg02955100 | −6.38 | 3.31e–10 | −0.007 (−1.02%) | PPT2-EGFL8 |
| cg07132926 | −6.38 | 3.40e–10 | −0.021 (−3.02%) | XPO4 |
| cg09194742 | −6.38 | 3.40e–10 | −0.01 (−1.35%) | HSPA12A |
| cg06516751 | −6.38 | 3.43e–10 | −0.022 (−3.79%) | WDR7 |
| cg25239226 | −6.37 | 3.49e–10 | −0.008 (−0.96%) | MLYCD |
| cg04576317 | −6.35 | 3.98e–10 | −0.007 (−0.96%) | DCAF11 |
| cg19613624 | −6.35 | 4.01e–10 | −0.005 (−0.71%) | PMEPA1 |
| cg04976850 | −6.35 | 4.17e–10 | −0.015 (−2.44%) | CLCA2 |
| cg20618167 | −6.34 | 4.25e–10 | −0.001 (−0.09%) | LOC101928103 |
| cg14468497 | −6.29 | 5.95e–10 | −0.014 (−2.55%) | RNF112 |
| cg02294570 | −6.29 | 5.96e–10 | −0.013 (−2.45%) | WDR66 |
| cg03230491 | −6.28 | 6.08e–10 | −0.008 (−1.05%) | CC2D1A |
| cg07790939 | −6.25 | 7.44e–10 | −0.012 (−1.45%) | SCIN |
| cg03057072 | −6.25 | 7.44e–10 | −0.005 (−0.72%) | ARHGAP12 |
| cg01220720 | −6.25 | 7.59e–10 | −0.008 (−1.1%) | NOTCH4 |
| cg13548652 | −6.24 | 7.84e–10 | −0.021 (−2.94%) | ZCCHC6 |
| cg21740507 | −6.23 | 8.67e–10 | −0.015 (−2.18%) | IQCA1 |
| cg20569791 | −6.22 | 8.79e–10 | −0.009 (−1.47%) | PLGRKT |
| cg22958188 | −6.22 | 9.32e–10 | −0.004 (−0.4%) | LINC02337 |
| cg26173773 | −6.21 | 9.39e–10 | −0.006 (−0.83%) | PTPRN2 |
| cg01216201 | −6.21 | 9.57e–10 | −0.006 (−0.73%) | CCDC57 |
| cg12213699 | −6.2 | 1.01e–09 | −0.015 (−1.89%) | SYNE2 |
| cg10988013 | −6.19 | 1.06e–09 | −0.015 (−2.12%) | PAPPA |
| cg01137782 | −6.19 | 1.11e–09 | −0.008 (−1.06%) | TIGIT |
| cg02641865 | −6.16 | 1.34e–09 | −0.02 (−2.79%) | RLF |
| cg19609713 | −6.15 | 1.34e–09 | −0.008 (−0.9%) | CD27-AS1 |
| cg08857906 | 6.14 | 1.44e–09 | 0.007 (1.64%) | PPP1R8 |
| cg02395863 | −6.13 | 1.59e–09 | −0.003 (−0.33%) | RGS7 |
| cg23131559 | −6.1 | 1.76e–09 | −0.008 (−1.18%) | GDPD3 |
| cg16933892 | −6.09 | 1.92e–09 | −0.006 (−0.88%) | PI3 |
| cg22486192 | −6.09 | 1.94e–09 | −0.017 (−2.52%) | MVP |
| cg25947600 | −6.08 | 2.06e–09 | −0.009 (−1.22%) | C19orf68 |
| cg05810476 | −6.06 | 2.28e–09 | −0.013 (−2.43%) | PTEN |
| cg16190127 | −6.06 | 2.40e–09 | −0.009 (−1.3%) | WDR27 |
| cg11748170 | −6.06 | 2.47e–09 | −0.01 (−1.21%) | NOTCH4 |
| cg02482718 | −6.05 | 2.58e–09 | −0.007 (−1.16%) | AJAP1 |
| cg13786513 | −6.04 | 2.64e–09 | −0.002 (−0.22%) | PPP1R37 |
| cg12165864 | −6.03 | 2.70e–09 | −0.008 (−0.92%) | LOC644794 |
| cg07367519 | −6.02 | 2.83e–09 | −0.002 (−0.22%) | CACNA1I |
| cg13486309 | −6.02 | 2.99e–09 | −0.015 (−2.12%) | ASH1L |
| cg09143779 | −6.01 | 3.15e–09 | −0.01 (−1.41%) | DSE |
| cg08314899 | −006 | 3.21e–09 | −0.013 (−1.77%) | MYBPC1 |
| cg19118037 | −006 | 3.25e–09 | −0.007 (−1.02%) | AIPL1 |
| cg01217923 | −5.93 | 5.12e–09 | −0.003 (−0.47%) | ZNF564 |
| cg03463293 | −5.93 | 5.14e–09 | −0.006 (−0.76%) | C15orf39 |
| cg25612428 | −5.89 | 6.16e–09 | −0.001 (−0.12%) | ANKRD33B |
| cg26222012 | −5.88 | 6.82e–09 | −0.001 (−0.15%) | PRSS37 |
| cg12660062 | −5.85 | 7.79e–09 | −0.003 (−0.49%) | LOC101926942 |
| cg15999796 | −5.79 | 1.13e–08 | −0.012 (−1.86%) | MOG |
| cg11162741 | −5.78 | 1.19e–08 | −0.015 (−2.21%) | TRAF1 |
| cg17736252 | −5.76 | 1.34e–08 | −0.005 (−0.62%) | MIGA1 |
| cg14500563 | −5.68 | 1.99e–08 | −0.007 (−0.84%) | MIR602 |
| cg05309750 | −5.67 | 2.13e–08 | −0.011 (−1.48%) | GXYLT2 |
| cg23863528 | −5.67 | 2.19e–08 | −0.011 (−1.52%) | GAB1 |
| cg14775469 | −5.58 | 3.49e–08 | −0.016 (−2.49%) | SFSWAP |
| cg11617879 | −5.58 | 3.60e–08 | −0.017 (−2.29%) | USP22 |
CpGs remaining to be significantly associated with anxiety disorders at p ≤ 6.409e–08 after statistical adjustment for comorbidity with depression (defined as diagnosis of major depressive disorder, single episode or recurrent); Δβ: Positive values indicate methylation in anxiety disorder patients > methylation in healthy controls, negative values indicate methylation in anxiety disorder patients < methylation in healthy controls.