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. 2002 Jun 18;99(13):8707–8712. doi: 10.1073/pnas.082248899

Figure 4.

Figure 4

Staging CDYL protein expression and histone H4 hyperacetylation in mouse spermatids. (A) Schematic depiction of the 16 steps of mouse spermiogenesis and the time window where CDYL protein expression and histone H4 hyperacetylation were detected. (B) A series of mouse seminiferous tubule squash preparations containing spermatids of steps 8–13 was immunohistochemically stained with anti-CDYL serum. It reveals CDYL immunoreactivity in step 9–12 spermatids. (C) A parallel series of squash preparations were stained with serum against AcH4. It shows AcH4 immunoreactivity in step 9–12 spermatids.