Reagents and tools table
| Reagent/resource | Reference or source | Identifier or catalog number |
|---|---|---|
| Experimental models | ||
| SPARCF/F mice | Shanghai Model Organisms Center, Inc. | NM-CKO-2109560 |
| Dnmt (1, 3a)2F/2F | Sun et al, 2020 | N/A |
| EP3−/− mice | Tang et al, 2017 | N/A |
| EP3F/FLysMCre mice | Tang et al, 2017 | N/A |
| Rosa-EP3α | Tao et al, 2022b | N/A |
| Recombinant DNA | ||
| PcDNA3.1-Sp1 | Kong et al, 2020 | N/A |
| pGL3-Dnmt1 | Hanheng Biotechnology | N/A |
| pGL3-Dnmt3a | Hanheng Biotechnology | N/A |
| Antibodies | ||
| Rabbit anti-Dnmt1 | Cell Signaling Technology | 5032 |
| Rabbit anti-Dnmt3a | Abcam | ab188470 |
| Rabbit anti-Dnmt3b | Abcam | ab2851 |
| Rabbit anti-SPARC | Cell Signaling Technology | 5420 |
| Rabbit anti-SP-1 | Proteintech | 21962-1-AP |
| Rabbit anti-phospho-SP1 (T453) | Abcam | ab59257 |
| Rabbit anti-Tet1 | Abclonal | A1506 |
| Rabbit anti-Tet2 | Proteintech | 21207-1-AP |
| Rabbit anti-Tet3 | Abclonal | A7612 |
| Rabbit anti-NF-κb p65 | Cell Signaling Technology | 8242S |
| Rabbit Histone H3 | Cell Signaling Technology | 4499S |
| Rabbit anti-NOS2 | Abcam | ab15323 |
| Goat anti-YM1 | R&D Systems | AF2446 |
| HRP-conjugated Rabbit anti-Goat IgG | Abclonal | AS029 |
| HRP-conjugated secondary antibodies | Cell Signaling Technology | 7074S, 7076S |
| Mac-3 | Biolegend | 108512 |
| CD31 | Abcam | ab28364 |
| Alexa Fluor 488 conjugated secondary antibodies | Invitrogen | A11008 |
| Alexa Fluor 647 conjugated secondary antibodies | Jackson ImmunoResearch | 112-606-07 |
| Anti-CD45-APCCy7 | ebioscience | 47-0451-82 |
| Anti-F4/80-Brilliant Violet 421 | BioLegend | 123137 |
| Anti-CD11b-FITC | BioLegend | 101206 |
| Oligonucleotides and other sequence-based reagents | ||
| siRNA sequences | This study | Appendix Table S1 |
| RT-qPCR Primers | This study | Appendix Table S2 |
| BSP methylation Primer | This study | Appendix Table S3 |
| Chemicals, enzymes and other reagents | ||
| Decitabine | Sigma-Aldrich | A3656 |
| Insulin | Sigma-Aldrich | I6634 |
| Collagenase II | Sigma-Aldrich | S20133006 |
| Dexamethasone | Sigma-Aldrich | D4902 |
| 3-Isobutyl-1-methylxanthine | Sigma-Aldrich | I5879 |
| Rosiglitazone | Sigma-Aldrich | D2408 |
| d-glucose | Sigma-Aldrich | G6152 |
| H-89 | Cayman | 10010556 |
| Mithramycin A | Cayman | 11434 |
| Lipofectamine™ RNAiMAX Transfection Reagent | Invitrogen | 13778075 |
| IL-6 ELISA Kit | E-EL-M0044 | Elabscience |
| TNF-α ELISA Kit | E-EL-M3063 | Elabscience |
| MCP-1 ELISA Kit | E-EL-M3001 | Elabscience |
| Mouse Peripheral Blood Mononuclear Cell Isolation Kit | P5230 | Solarbio |
| Palmitate Acid | KC002 | Kunchuang Biotechnology |
| TRIzol reagent | 15596018 | Invitrogen |
| Hifair® III 1st Strand cDNA Synthesis SuperMix for qPCR (gDNA digester plus) | 11141ES60 | YeaSen Biotechnology |
| Oil Red O staining | G1015 | Servicebio |
| Software | ||
| ImageJ | https://imagej.net/ | |
| GraphPad Prism 8.0 | https://www.graphpad.com | |
| Signal P 4.1 | http://www.cbs.dtu.dk/services/SignalP/ | |
| TMHMM server 2.0 | http://www.cbs.dtu.dk/services/TMHMM/ | |
| Secretome P 2.0 | http://www.cbs.dtu.dk/services/SecretomeP/ | |
| DAVID | https://davidbioinformatics.nih.gov/ | |
| Other | ||
| FACSAriaTM II flow cytometry system | BD Biosciences | |
| TimeTOF Pro mass spectrometer | Bruker | |
| ACQUITY UPLC liquid chromatography system | Waters | |
| 5500 QTRAP mass spectrometer | AB Sciex | |
| Laser confocal ultra-high-resolution microscope | ZEISS | |
| LightCycler 480 Instrument II | Roche | |
| The metabolic chambers of the CLAMS | Columbus | |
| Transwell system | Corning | |