Reagents and tools table

Reagent/resource	Reference or source	Identifier or catalog number
Experimental models
C57Bl6/J (M. musculus)	Janvier Labs	C57BL/6-Jrj
Swiss (M. musculus)	Janvier Labs	RjOrl:SWISS (CD-1)
Recombinant DNA
pRN3-H2B-mCherry-hScc1(107-268)-YFP	This study	Nikalayevich et al (2022)
pRN3-mseparase	Kudo et al (2006)
pRN3-mseparase S1121A	Touati et al (2012)
pRN3-msecurin	Touati et al (2012)
pRN3-human Cyclin B1(MRL)-mGFP	This study
Antibodies
Human anti-centromere protein (ACA)	Clinisciences	15-234
Human CREST serum	Immunovision	HCT-100
Rabbit anti-Rec8	Gryaznova et al (2021)
Rabbit anti-Sgo2	This study
Mouse anti-PP2AC-1D6-Alexa Fluor 488	Sigma-Aldrich	05-421-AF488
Rabbit anti-Mad2	Wassmann and Benezra, (1998)
Donkey anti-human CY3	Jackson Immuno Research	709-166-149
Donkey anti-human Alexa Fluor 647	Jackson Immuno Research	709-606-149
Donkey anti-rabbit CY3	Jackson Immuno Research	711-166-152
Donkey anti-rabbit Alexa Fluor 488	Jackson Immuno Research	711-546-152
Donkey anti-mouse Alexa Fluor 488	Jackson Immuno Research	715-546-150
Chemicals, enzymes, and other reagents
dibutyryl cyclic AMP	Sigma-Aldrich	D0260
Embryo culture oil	FUJIFILM Irvine Scientific	9305
Strontium chloride hexahydrate	Sigma-Aldrich	204463
Paraformaldehyde	Sigma-Aldrich	441244
Triton X-100	Sigma-Aldrich	T8787
Dithiothreitol (DTT)	Sigma-Aldrich	D9779
Bovine Serum Albumin (BSA)	Sigma-Aldrich	A4378
Vectashield DAPI	EUROBIO Scientific	H-1200
SiR-DNA	Spirochrome	SC007
Nocodazole	Sigma-Aldrich	M1404
Software
Metamorph 7.10.3	Metamoprh	https://support.moleculardevices.com/s/article/MetaMorph-Software-installation-files
Fiji 5.1.0	Fiji	https://imagej.net/software/fiji/
PRISM 10	GraphPad	https://www.graphpad.com/scientific-software/prism/
Other
mMessage mMachine T3 kit	Invitrogen	AM1348
RNeasy Mini Kit 5	Qiagen	74104