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. 2002 Jun 11;99(13):8772–8777. doi: 10.1073/pnas.132275999

Figure 2.

Figure 2

Number of MTX-resistant subclones with each type of palindrome analyzed in all transformants. Nine, eight, and four transformants of noIR, 79IR, and 229IR respectively were subjected to DSB induction and MTX selection. The number of MTX-resistant subclones from cells with DSB (Right) and without DSB (Left) are indicated by the lengths of the bars. The presence of various types of palindromes in the MTX-resistant subclones of every transformant was determined by Southern hybridization analysis, and their fractions are indicated by using different shadings. Many subclones from transformants 79IR-2, -8, and -28 contained short IR-mediated palindromes. 229IR-16 and -17 contained only IR-mediated palindromes. Transformants of noIR produced a lower number of MTX-resistant subclones than 79IR transformants (79IR+DSB vs. noIR+DSB, P = 0.02). The “others” category includes subclones with unrearranged DNA, other types of rearranged DNA, or mixtures of DNA types that might include palindromes.