Skip to main content
. 2002 Jun 19;99(13):8796–8801. doi: 10.1073/pnas.132270899

Figure 2.

Figure 2

Steady-state analysis of GAL constructs. Northern blot with total RNA from pYC10-7 (WT, lane 1), pYC10-7Con (Con, lane 2), pYC10-7Fus (Fus, lane 3), Fus-Δ7P (lane 4), Fus-Δ10P (lane 5), Fus-Δ7P/Δ10P (lane 6), and Fus-Δ7P&Δ10P (lane 7). Longer exposure shows the read-around bands for Fus-Δ7P (lane 4) and Fus-Δ10P (lane 5). The filter was striped and reprobed with ACT1 as a loading control. Quantitated levels of steady-state GAL10 and GAL7 mRNA are corrected for actin and compared by using the steady-state level of GAL10 and GAL7 mRNA from WT as 1.