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. 2002 Jun 19;99(13):8796–8801. doi: 10.1073/pnas.132270899

Figure 4.

Figure 4

Quantitation of Fus and Fus-Δ7P and Fus-Δ10P transcription profiles. (A) TRO analysis of Fus transformed plasmid. The signals were corrected as described for Fig. 1. Arrows indicate the directions of transcribing polymerases. (B) TRO analysis of Fus-Δ7P and Fus-Δ10P. In the Fus-Δ10P plasmid (giving GAL7 transcription), the O probes have been deleted with the deletion of the GAL10 promoter. In the GAL10 transcription profile, decrease in GAL10 transcription over probe A is likely the result of cryptic polyadenylation that is seen at the steady-state level (Fig. 2, lane 4). Also, the above background levels of transcription over probe O (before the GAL10 promoter) are most likely to be the result of read-around transcription seen at steady state (Fig. 2, lane 4). The single-stranded M13 probes used are described in Table 1.