Table 1.
CD4+CD25+ T cells reduce the number of CD8+ memory cells in mice mainly by acting on dividing cells
| Experiment | Recipient | Cells transferred into T cell-deficient recipients
|
Number (×10−4) of CFSE+CD8+ memory T cells recovered from animals that received
|
Change in yield of CFSE+CD8+ memory T cells in the absence/presence of CD4+CD25+ T cells
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Filler cells lacking CD4+CD25+ cells
|
Number (×10−7) CFSE+ indicator cells | Number (×10−6) CD25+ or CD25− test cells | CD4+CD25− test T cells
|
CD4+CD25+ test T cells
|
|||||||
| Origin | Number (×10−7) | Nondividing | Dividing | Nondividing | Dividing | Nondividing | Dividing | ||||
| 1 | RagKO | CD25KO | 7 | 3 | 1.7 | 3.1 | 37 | 2.6 | 8.5 | 1.19 | 4.35 |
| 2 | RagKO | CD25KO | 7.5 | 2.5 | 2 | 4.9 | 7.4 | 3.2 | 2.7 | 1.53 | 2.74 |
| 3 | RagKO | Sorted | 1 | 8 | 0.5 | 5.2 | 64 | 4.2 | 8.1 | 1.24 | 7.90 |
| 4 | Irradiated | Sorted | 2.7 | 6.3 | 2 | 4.3 | 6.9 | 10 | 8.2 | 0.43 | 0.84 |
| 5 | RagKO | Anti-CD25 | 13 | 6 | 1.3 | 9.6 | 12.7 | 2.4 | 3.1 | 4.00 | 4.10 |
| 6 | Irradiated | Anti-CD4 | 7.5 | 2.3 | 1 | 9.9 | 22.5 | 5.4 | 5.5 | 1.83 | 4.09 |
Recovered memory cell numbers are those of the CD44high or IL-2Rβhigh CD8+CFSE+ cells in the spleen and inguinal, brachial, and mesenteric lymph nodes of recipients.