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. 2002 Jun 19;99(13):8874–8879. doi: 10.1073/pnas.132269299

Figure 5.

Figure 5

C3 Tg lacking ex 6 and 15 are developmentally immature. (A) Cross sections of Tg and non-Tg soleus muscles stained for NCAM (red) and counterstained with hematoxylin (blue). NCAM is normally concentrated at the neuromuscular junction in adult tissue. In the ex 6− Tg, and in the ex 15− Tg soleus, NCAM is distributed around the sarcolemma in many of the fibers. (B) Cross sections of Tg and non-Tg gastrocnemius and soleus stained for AchRα. Note the distribution of AchRα in normal vs. ex 6− and ex 15− Tg is similar to that of NCAM. (C) Cross sections of non-Tg and Tg soleus muscles stained for slow MHC (red) and counterstained with hematoxylin (blue). Inset of ex 6− Tg shows an area of gastrocnemius muscle that is positive for slow MHC (red). This area probably represents new myofiber growth. Arrowheads show fibers with strong phenotype that do not stain for slow MHC.