Table 3:
Transformed strains used to evaluate ncAA incorporation efficiency in combination with gene knockouts.
| Strain (-HIS, -LEU, -URA) |
Reporter (URA) |
eRF1 Variant (LEU) |
|---|---|---|
| BY4741 | pRS416-BXGAltTAG-TyrOmeRS | WT SUP45 |
| BY4741 | pRS416-BXGAltTAG-TyrOmeRS | S30P |
| BY4741 | pRS416-BXGAltTAG-TyrOmeRS | BR3T.4 |
| BY4741 | pRS416-BY G-TyrOmeRS | WT SUP45 |
| BY4741 | pRS416-BY G-TyrOmeRS | S30P |
| BY4741 | pRS416-BY G-TyrOmeRS | BR3T.4 |
| BY474MPPQ1 | pRS416-BXGAltTAG-TyrOmeRS | WT SUP45 |
| BY4741ΔPPQ1 | pRS416-BXGAltTAG-TyrOmeRS | S30P |
| BY4741ΔPPQ1 | pRS416-BXGAltTAG-TyrOmeRS | BR3T.4 |
| BY474MPPQ1 | pRS416-BY G-TyrOmeRS | WT SUP45 |
| BY4741ΔPPQ1 | pRS416-BY G-TyrOmeRS | S30P |
| BY4741ΔPPQ1 | pRS416-BY G-TyrOmeRS | BR3T.4 |
| BY4741ΔYIL014C-A | pRS416-BXGAltTAG-TyrOmeRS | WT SUP45 |
| BY4741ΔYIL014C-A | pRS416-BXGAltTAG-TyrOmeRS | S30P |
| BY474MYIL014C-A | pRS416-BXGAltTAG-TyrOmeRS | BR3T.4 |
| BY4741ΔYIL014C-A | pRS416-BY G-TyrOmeRS | WT SUP45 |
| BY4741ΔYIL014C-A | pRS416-BY G-TyrOmeRS | S30P |
| BY4741ΔYIL014C-A | pRS416-BY G-TyrOmeRS | BR3T.4 |