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. 2025 Sep 2;21(9):e1013454. doi: 10.1371/journal.ppat.1013454

Fig 7. Enrichment of HPV31 trans-interactions at HPV integration hotspots.

Fig 7

HPV31 association sites within the host genome were correlated with sites of recurrent integration (hotspots) defined in different cervical tumors [17]. A, Table showing the regions of overlap between HPV31 trans-interactions mapped through HiC (n = 87 peaks) and 4C-seq (n = 371 consensus peaks) with sites of recurrent HPV DNA integration (n = 36 hotspots). Permutation testing showed significant overlap of HiC peaks and 4C consensus peaks with integration hotspots. B, Venn diagram showing the number of integration hotspots (n = 36) that overlap with HiC peaks and 4C consensus peaks. C, Bar chart showing the association of integration breakpoints with A/B compartments grouped as indicated in the table in Fig 5D. D, Circos plot showing HPV31 association sites with host chromosomes. Blue lines represent viral-host trans-interactions identified by 4C-seq (consensus peaks); red lines mark consensus sites also identified by HiC (n = 28). Bootstrap analysis showed HPV31 trans-interactions to be significantly enriched at super-enhancers and integration hotspots. E, Alignment of viewpoint 3 4C peaks (light blue peaks) to the HPV integration hotspot (light green bars) at the MYC locus on chr8. Consensus peaks are marked by the grey bars; the red bar indicates a HiC consensus peak. Super-enhancers (SE) are indicated by dark green bars and ATAC-seq signal by dark blue peaks.