Table 1.
CpG expression frequency in HSV-1 vs. murine DNA
| Motif | Expression frequency
|
Fold difference | ||
|---|---|---|---|---|
| E. coli | Mouse | HSV-1 | ||
| GACGTT | 1.3 | 0.11 | 0.82 | 7.5 |
| AGCGTT | 1.7 | 0.17 | 0.46 | 2.7 |
| AACGTC | 0.6 | 0.11 | 0.81 | 7.4 |
| AGCGTC | 1.3 | 0.15 | 0.95 | 6.3 |
| GGCGTC | 1.4 | 0.15 | 4.50 | 30.0 |
| GGCGTT | 2.5 | 0.15 | 1.68 | 11.2 |
| Average | 1.53 | 0.14 | 1.54 | 11.0 |
The frequency with which each CpG hexamer is expressed in the genome of Escherichia coli, mice and HSV-1 was determined by using published sequence data. Accession numbers are from the National Center for Biotechnology Institute. (The mouse chromosome data were a composite of chromosomes 1–3. The accession numbers for mouse chromosomes 1, 2, and 3 are: NT_025524, NT_019187, and NT_015485. The accession numbers for E. coli-K12 and HSV-1 complete genome are NC_000913 and X14112.) Note the significant overexpression of immunostimulatory GCGTC and GCGTT motifs in the HSV genome vs. the expression of the mouse (fold difference).