Skip to main content
. 2002 Jun 11;99(13):8962–8967. doi: 10.1073/pnas.102285099

Figure 5.

Figure 5

Insertional inactivation of vanAst. (A) Orientation of the apramycin-resistance cassette in the resistance cluster. (B) A47934 inhibits growth of the vanAST∷Am disruption mutant but not wild-type S. toyocaensis. The sensitivity can be reversed by supplying the vanH,A,X cluster in trans on pJN7. (C) Liquid culture growth of the vanAST∷Am S. toyocaensis mutant (●) shows no difference with wild-type S. toyocaensis (○). However, antibiotic production is delayed approximately 16 h in the disruption strain (■) vs. wild type (□).