Table 1.

Summary of proteins encoded by the A47934 biosynthetic gene cluster

ORF	Start/stop, bp	A47934 Protein	Cep homologue*	Com homologue†	Bal homologue‡	Proposed function/homology
1	914–288	VanXst	—	—	—	d-Ala-d-Ala dipeptidase
2	1951–911	VanAst§	—	—	—	d-Ala-d-lactate ligase
3	2931–1939	VanHst	—	—	—	Lactate dehydrogenase
4	3059–4399	MurX	—	—	—	d-Ala-d-Ala adding enzyme
5	5578–4412	StaO	—	—	—	FemABX homologue
6	6509–5667	StaP	—	—	—	Putative membrane protein
7	8101–9102	StaQ	—	ComG	—	Transcriptional regulator
8	10324–9224	Hmo	Hmo	Hmo	ORF 6	p-Hydroxymandelate oxidase
9	11417–10308	HmaS	HmaS	HmaS	ORF 5	p-Hydroxymandelate synthetase
10	11592–12746	Pdh	Pdh	Pdh	—	Prephenate dehydrogenase
11	12908–14266	HpgT	HpgT	HpgT	PgaT	p-Hydroxy- and 3,5-dihydroxyphenylglycine aminotransferase
12	14412–15584	DpgA	DpgA	—	DpgA	3,5-Dihydroxyphenylacetyl-CoA synthase
13	15596–16273	DpgB	DpgB	—	DpgB	Enhances DpgA activity
14	16270–17586	DpgC	DpgC	—	DpgC	3,5-Dihydroxyphenylacetyl-CoA oxygenase
15	17583–18386	DpgD	DpgD	—	DpgD	Enhances DpgA activity
16	21475–18767	StaR	—	—	—	Putative flavoprotein
17	21820–22284	StaS	—	—	—	Putative DNA binding protein
18	23466–22363	VanSst	—	—	—	Transmembrane histidine kinase
19	24148–23453	VanRst	—	—	—	Two-domain response regulator
20	24407–26350	StaU	CepM	ComL	—	ABC transporter
21	26347–32559	StaA	CepA	ComA	BpsA	Peptide synthetase (modules 1–2)
22	32562–37037	StaB	CepA	ComB	BpsA	Peptide synthetase (module 3)
23	37067–49267	StaC	CepB	ComC	BpsB	Peptide synthetase (modules 4–6)
24	49287–54854	StaD	CepC	ComD	BpsC	Peptide synthetase (module 7)
25	55192–56367	StaE	CepD	ComE	ORF1	Hypothetical protein
26	55192–56367	StaF	CepE	ComI	OxyA	P450-related oxidase
27	56388–57542	StaG	—	—	—	P450-related oxidase
28	57532–58728	StaH	CepF	ComJ	OxyB	P450-related oxidase
29	58733–60271	StaI	CepH	—	BhaA	Nonheme halogenase
30	60315–61493	StaJ	CepG	—	OxyC	P450-related oxidase
31	61538–62845	StaK	—	ComH	—	Nonheme halogenase
32	62842–63654	StaL	—	—	—	Sulfotransferase
33	63734–65320	StaM	—	—	—	Putative nonheme iron dioxygenase
34	65783–67039	StaN	CZA382.28	ComF	—	Integral membrane ion transporter

^*Cep proteins from the chloroeremomycin producer A. orientalis A82846. 

^† Com proteins from the complestatin producer Streptomyces lavendulae. 

^‡ Bal proteins from the balhymicin producer Amycolatopsis mediterranei DSM5908. 

^§ Previously identified as DdlM (13, 41).