Figure 6. AAV-mediated knockdown of hepatic HMGCS2 causes mitochondrial calcium overload inversely correlated with state 3 mitochondrial respiration.
A and B, canonical signalling pathways revealed from proteomic (A) and phosphor-proteomic (B) IPA analyses. Blue indicates a decrease and orange an increase in HMGCS2-KD compared with SCR-SED rats. C, ADP/ATP translocase 2 (ANT2) protein expression in the frontal cortex expressed as raw intensity (n = 6). D, single-pass membrane protein with aspartate-rich tail (SMDT1) protein expression in the frontal cortex expressed as raw intensity (n = 6). E, correlation between State 3 Complex I + II mitochondrial respiration and SMDAT1 raw intensity in HMGCS2 KD-SED and SCR-SED rats. Z-score generated in IPA. Values presented as mean ± SD.