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. 2002 Jun 11;99(13):9010–9014. doi: 10.1073/pnas.132260399

Figure 1.

Figure 1

Uptake of SOD1 and swelling of mouse mitochondria. (A) Uptake of SOD1 by mouse liver mitochondria. Isolated mitochondria were incubated with holo or demetallated SOD1 and then analyzed for content of SOD1 by immunoblotting as described in Materials and Methods. CuZn, holo enzyme; EE, apo; CuE, SOD1 lacking Zn; EZn, SOD1 lacking Cu. Each lane was loaded with 10 μg of protein. (B) Mitochondrial swelling. Suspensions of mitochondria in the MRM medium at 25°C were incubated ± bongkrekic acid at 5 μM or alamethicin at 40 μg/ml and swelling was followed at 540 nm.