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Effect of cytosol and Hsp70 on uptake of SOD1 into N2A mitochondria. (A) Mitochondrial uptake of SOD1. (B) Effect of Hsps. The uptake of SOD1 by mitochondria isolated from N2A cells was examined as in Fig. 1 in the absence and presence of cytosolic fractions. (A) In the absence of cytosol fraction. (B) In the presence of cytosol, cytosol from heat-shocked cells, or 1 μg Hsp70 (StressGen). CuZn, holo enzyme; EE, apo; CuE, SOD1 lacking Zn; EZn, SOD1 lacking Cu.
