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. 2002 Jun 11;99(13):9010–9014. doi: 10.1073/pnas.132260399

Figure 4.

Figure 4

Preferential binding of mutant SOD1 by Hsp70 and Hsp25. (A) Induction of Hsp25 and Hsp70. (B) Mutant SODs bind Hsps. Binding of mutant SOD1 and Hsps was examined by immunoprecipitation (IP) and immunoblotting (IB). Total cell lysate from normal or heat-shocked mouse N2A cells that permanently transfected human WT, G37R, or G41D SOD1 was examined before (total) and after (sup) centrifugal clarification by immunoprecipitation. (A) Immunoprecipitation and immunoblotting used with anti-Hsp25 or anti-Hsp70 antibody. (B) Immunoprecipitation used with anti-Hsp25 or anti-Hsp70 antibody, and immunoblotting used with anti-SOD1 antibody.