Figure 1.

Mutation of the murine Munc13-2 gene. (A) Maps of the wild-type Munc13-2 gene, the respective targeting vector, and the resulting mutant gene. Positions of exons (black boxes) and restriction enzyme sites are indicated. Hatched bar, Position of probe used for Southern. Neo, Neomycin resistance gene; TK, thymidine kinase gene. (B) Southern blot analysis of the Munc13-2 KO mutations in mice by using mouse tail DNA (EcoRI digest) from the indicated Munc13-2 genotypes. (C) Munc13-2 protein expression in mice of the indicated genotypes as determined by Western blotting using an antibody that recognizes all Munc13-2 splice variants. (D) Expression of Munc13-1 and Munc13-2 protein in mice of the indicated genotypes as determined by Western blotting using an antibody to Munc13-1 (Upper) and an antibody that recognizes all Munc13-2 splice variants (Lower).