Table 1. Bulk and single cell RNA sequencing datasets of human tissues.

Study	Phenotype	Genotype	Sample size	Sequencing type
I. GEO: GSE142538	case	RE+	6	bulk
		RE-	4
	control	RE+	6
		RE-	9
II. SRA: PRJNA524323	case	RE+	8	bulk
		RE-	4
	control	RE-	6
III. GEO: GSE112201	case	RE+	11	bulk
	control	RE-	4
IV. GEO: GSE155683	Unknown	Unknown	4	single cell

Phenotype is defined in terms of Fuchs’ endothelial corneal dystrophy affection status. Genotype RE+ and RE- refer to with and without the TCF4 CTG18.1 expansion allele ($\ge 40$ repeats). The bulk and single cell RNA-seq experiments were performed using human corneal endothelium and cornea-conjunctival tissues, respectively. All bulk RNA-seq samples were sequenced with paired-end reads on an Illumina platform with read length of 150, 125, and 101 bp in I, II, and III, respectively. The single cell RNA-seq libraries were prepared using Chromium Single Cell 3′ Library & Gel Bead Kit (v3, 10x Genomics) and sequenced on an Illumina NovaSeq 6000.