Table 4.
PCR validation primers designed based on rDNA high-throughput sequencing and complete mitochondrial genome sequencing results.
| Primer ID | Sequence (5’-3’) | Gene | Product size (bp) |
|---|---|---|---|
| W1724f | GCTACACTGACAGAGCCAACG | rDNA | 473 |
| W2196r | TGAAACTCCGACGCAAAGA | ||
| A1956f | TCGGCAACGACCACCCA | ITS | 730 |
| A2685r | CTACCCAGAAGCATCCTCTACAAA | ||
| 1w7393F | CACTGCTTCTGCTTTCTTTT | Cyt b | 490 |
| 1w7882R | TATTAAATAGACACCACTTC | ||
| 2w25369F | ATAACCGAGGTAGTCAGAGC | COI | 700 |
| 2w26068R | CTTGTAGTGTATCTGTGTAA |
Complete mitochondrial genome of Pseudocercospora mori has been submitted to NCBI with accession number: MG543071.1.
W1724f/W2196r are primers for specific detection of Pseudocercospora mori; A1956f/A2685r are primers for amplification of fungal ITS region; 1H21668F/1H22222R, 2H13208F/2H13744R, 3H15185F/3H15896R, 5H5452F/5H6077R are primers for specific detection of Pseudocercospora mori; 1w7393F/1w7882R and 2w25369F/2w26068R are for mitochondrial Cyt b and COI genes partial segment amplification, respectively.