FIGURE 2.

Expression of a promoter‐less Scarlet cassette driven by the endogenous shROSA26 promoter. (a) Strategy for promoter‐less gene cassette KI at the shROSA26 locus. PCR primer locations are indicated by arrows. (b) Images of E12.5 Scarlet‐positive and negative embryos. Bright‐field image (left) and RFP fluorescence image (right). Scale bar: 4 mm. (c) Genomic PCR analysis of a wild‐type and a Scarlet‐positive embryo. Blue and red arrows indicate the positions of the 5′ and 3′ KI bands, respectively. M, DNA ladder marker.