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. 2002 May 14;99(10):6649–6654. doi: 10.1073/pnas.102523299

Figure 1.

Figure 1

PRDX3 is regulated by c-myc expression. (A) RNA from Rat1a (R1a) fibroblasts or Rat1a fibroblasts expressing ectopic c-Myc (R1a-myc). rpL32 is shown as a loading control. RNA was isolated from adherent cells (A) or nonadherent cells grown over a layer of agar (N). (B) PRDX3 expression in logarithmically growing c-myc+/+, +/−, or −/− Rat1 fibroblasts. PRDX3 expression was calculated relative to vimentin. (C) Hepatic RNA from mice injected with either adenoviral LacZ or c-myc. Numbers represent days after injection with adenovirus. 18S RNA is shown as a loading control. (D) Schematic representation of the PRDX3 genomic locus. Exons are indicated by black boxes. Fragments analyzed for Myc binding are indicated by lettered black bars. The sole canonical E box is indicated in bold, and noncanonical E boxes (38, 39) in fragments C and D are also shown. (E) Ethidium bromide-stained gels of PCR products. (F) Sybr green analysis of PCR fragments evaluated for Myc binding. The absolute amount of DNA in each sample was calculated, and the average was plotted ± SD. (G) Relative mRNA levels for c-myc and PRDX3 during serum stimulation. Signals were normalized to the level of 18S RNA and plotted relative to the 0 h time point for each series.