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Immunoblot and immunolocalization with suNCKX antibody. (A) Lane 1 is a silver stained gel of total sperm membrane vesicle protein; lane 2 is the same sample reacted with anti-P1 antibody. Relative molecular masses are on left; 2.5 μg protein on lane 1, and 5 μg on lane 2. (B) Immunolocalization of suNCKX with anti-P1 antibody. (Left) Phase contrast image; (Right) immunofluorescence. The width of each sperm head is ≈1 μm.
