Sequence analysis of GbpC and -D. Residues are shaded black if they are
identical to the consensus for each position in the alignment, and
shaded gray if they are similar to the consensus. (A)
Alignment of the Ras sequences from GbpC, the Drosophila
CG5483 protein (D.m., GenBank accession no. AAF55793), a
human protein encoded by the KIAA1790 cDNA (H.s.,
BAB47419), and human H-Ras (P01112). Key residues involved in GTP
hydrolysis are highlighted and marked with an asterisk.
(B) Alignment of the protein kinase sequences from GbpC,
transforming growth factor β-activated protein kinase 1 (Tak1,
O43318), a RAF homolog from Arabidopsis thaliana (Ctr1,
A45178), and mixed lineage kinase 1 (Mlk1, AAG44591). The twelve nearly
invariant protein kinase residues (41) are highlighted and indicated
with an asterisk. For positions that show a conserved residue in >90%
of Tyr kinases, but not Ser/Thr kinases (42), the preferred amino
acid is indicated above the alignment. (C) Alignment of
the RasGEF catalytic domains from GbpC and -D with those from human
Sos-1 (Q07889) and Saccharomyces cerevisiae cdc25
(P04821). The bar above the alignment in C indicates the
helical hairpin involved in nucleotide exchange. (D)
Alignment of the DEP domain from GbpC with mouse
dishevelled protein (Dv1, P51141), mouse pleckstrin
(Plk., AAG29513), and C. elegans Egl-10 (Egl10, P49809).
Residues implicated in forming a β-hairpin arm and electric dipole
are highlighted and indicated by asterisks.