Figure 3.
Localization of MinD K16Q in vivo. The localization of GFP-MinD was compared with GFP-MinD K16Q. (A) JS964 (Δmin) containing pZH106 (gfp-minD) was induced for 2 h with 0.01% arabinose and photographed. Two cells are displayed. (B) JS964 (Δmin) containing pZH106–16 (gfp-minD K16Q) was induced with 0.01% arabinose and examined by fluorescence microscopy 2 h later. Two cells are displayed. Immunoblot analysis demonstrated that the level of GFP-MinD and GFP-MinD K16Q were similar at 2 h after induction (data not shown).