Table 1.
Bacterial species in semen samples from 20 individual bulls (1–20) of Viking Holstein (VH) and Viking Red (VR) collected at three samplings occasions and identified by MALDI-TOF MS.
| Bacterial species | 1st ejaculate | 2nd ejaculate | 3rd ejaculate | Breed |
|---|---|---|---|---|
| Alkalihalobacillus clausii§ | 2 | VH | ||
| Actinobacillus seminis§ | 3 | VH | ||
| Bacillus cereus | 15 | 1, 7, 14 | VH/VR | |
| Bacillus licheniformis* | 6, 9 | 1, 3, 9, 14 | 5, 6, 8, 9, 18 | VH/VR |
| Bacillus pumilus* | 2, 5, 9, 11, 19 | 2, 5, 8, 14, 17 | 2, 5, 7, 8, 9, 16, 17 | VH/VR |
| Bacillus subtilis | 1 | 10 | VH/VR | |
| Brevibacillus parabrevis | 1 | 10 | VH/VR | |
| Corynebacterium cystitidis | 5 | 8 | VH/VR | |
| Corynebacterium freneyi§ | 10 | VR | ||
| Corynebacterium xerosis* | 3 | 3, 17 | 1 | VH |
| Enterobacter bugandensis | 8 | 6 | VH/VR | |
| Enterobacter cloacae* | 3, 11 | 8 | 8, 11 | VH/VR |
| Enterobacter ludwigii | 7 | 6 | VH | |
| Escherichia coli* | 9 | 18 | 9 | VR |
| Histophilus somni§ | 6, 18 | VH/VR | ||
| Micrococcus lylae§ | 3 | VH | ||
| Micrococcus luteus | 2 | 1 | VH | |
| Neisseria elongata§ | 10 | VR | ||
| Neisseria subflava§ | 8 | VR | ||
| Proteus hauseri§ | 16 | VR | ||
| Proteus mirabilis* | 13, 20 | 20 | 15, 20 | VR |
| Proteus vulgaris | 16 | 11 | VR | |
| Serratia liquefaciens | 5 | 9 | VH/VR | |
| Serratia rubidaea* | 1, 19 | 1, 9 | 1, 19 | VH/VR |
| Staphylococcus chromogenes* | 1, 3, 5, 9 | 1, 3, 5, 8 | 5, 10 | VH/VR |
| Staphylococcus epidermidis | 6, 12 | VH/VR | ||
| Staphylococcus pasteuri§ | 8 | VR | ||
| Staphylococcus sciuri* | 9, 15 | 2, 7 | 2, 6, 12, 14 | VH/VR |
| Staphylococcus warneri§ | 2, 4, 6, 12 | VH/VR | ||
| Streptococcus dysgalactiae§ | 17 | VR | ||
| Streptococcus oralis§ | 12 | VR | ||
| Streptococcus uberis§ | 18 | VR | ||
| Trueperella pyogenes§ | 17 | VR |
* Bacterial species identified across all ejaculates, § represent bacterial species that were identified on only one occasion.