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. 2025 Sep 5;12:1583136. doi: 10.3389/fvets.2025.1583136

Table 2.

Number of identified bacterial isolates (MALDI TOF MS) and AVSs (16S Sequencing) across different semen collection time points and bull breed.

MALDI TOF MS 16S sequencing
Time
T1 3 1,285
T2 7 988
T3 5 1,157
T1/T2 2 92
T1/T3 4 125
T2/T3 0 183
T1/T2/T3 9 334
Breed
VH 6 1,027
VR 12 2,607
VH/VR 15 339

*T1 – first ejaculate 96 h after previous collection, T2 – second ejaculates were collected 48 h after the first, T3 – third ejaculates were collected 24 h after the second. VH – Viking Holstein, VR – Viking red.