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. 2025 Sep 19;11(38):eadw4124. doi: 10.1126/sciadv.adw4124

Fig. 6. HTT regulates axonal growth through its actin bundling activity.

Fig. 6.

(A) Representative confocal images of Cos7 cells transfected with HTT_B-GFP (top), Basic1mut-GFP (middle), and Basic2mut-GFP (bottom) and immunostained with phalloidin (cyan) to visualize F-actin. The yellow bar drawn in the x/y section bottom images denotes the position where a single z section (top image) is generated. (B) Top: Representative confocal images of neurons expressing the indicated constructs. Bottom: Length at DIV4, ****P < 0.0001, one-way ANOVA with Dunn’s multiple comparisons followed by Kruskal-Wallis test. (Mean of difference between control and shHTT, 219.2; between control and shHTT + HTT_C domain, 122.6; between control and shHTT + HTT_NB domain, −23.78; between control and HTT_C domain, −35.36; between control and HTT_NB domain, −13.84; two independent experiments, at least 50 axons per condition and experiment.)