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. 2002 Apr 30;99(10):6816–6821. doi: 10.1073/pnas.092130099

Figure 1.

Figure 1

Generation of a GRIP1-deficient mouse strain. (A) Schematic representation of GRIP1 gene-targeting strategy. Restriction map of the wt GRIP1 locus, targeting vector, and targeted locus. The targeting vector contains a tau–LacZ reporter gene fused in-frame with the exon encoding the first PDZ domain. Antibodies used for Western blot analysis are indicated. (B) Genotypic analysis of E12 embryos by PCR. To identify wt and targeted loci, primers were designed in the deleted wt locus and the β-galactosidase region, respectively. (C) Expression of GRIP1 was assessed by Western blot analysis of protein extracts from E12 embryos. The antibody used to detect GRIP1 expression is specific for the C-terminal portion of GRIP1 and does not cross-react with GRIP2 (18). The 100-kDa cross-reactive band bound by the C-terminal peptide-specific antibody was not recognized by antisera raised to the PDZ4 or the PDZ7 domains of GRIP1 (data not shown) and is therefore not GRIP1 specific.