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. 2002 May 7;99(10):6866–6870. doi: 10.1073/pnas.102165099

Figure 2.

Figure 2

Western blot analysis of RanGAP expression in testes. Each lane contains the total protein from two pairs of testes boiled in SDS sample buffer, separated electrophoretically, transferred onto poly(vinylidene difluoride)-plus membrane, and probed with anti-RanGAP antibody. (A) Expression of RanGAP from the β2-tubulin promoter results in an approximate 20-fold increase in RanGAP expression. Lanes 1 and 2, Two independent transgenic lines expressing β2-RanGAP; lanes 3 and 4, two independent transgenic lines expressing β2-RanGAP R87A; lane 5, Cantons-S (control). (B) Expression of RanGAP is not increased in males carrying extra doses of E(SD). Lane 1, Males carrying two doses of E(SD); lane 2, males carrying one dose of E(SD); lane 3, Canton-S (control) males lacking E(SD).