Table 2.
Germ-line overexpression of Ran or RanGEF suppresses segregation distortion caused by wild-type RanGAP
| Transgene | SD-5R7/Rspss cn; Sd-RanGAP [E(SD) Rspi M(SD) St(SD)/Rspss; Sd-RanGAP] | SD-5R7/Rspss cn; β2-RanGAP [E(SD) Rspi M(SD) St(SD)/Rspss; β2-RanGAP] | SD-5R7/ReR-5 cn [E(SD) Rspi M(SD) St(SD)/E(SD) Rsps] |
|---|---|---|---|
| — | 1.0 ± 0.00 (n = 1322) | 0.933 ± 0.031 (n = 1637) | 0.740 ± 0.014 (n = 1947) |
| β2-Ran | 0.472 ± 0.066 (n = 768) | 0.444 ± 0.059 (n = 860) | 0.573 ± 0.032 (n = 1816) |
| β2-RanGEF | 0.518 ± 0.055 (n = 764) | 0.412 ± 0.045 (n = 773) | 0.562 ± 0.037 (n = 1231) |
Segregation ratios were determined in males expressing Ran or RanGEF driven from the β2-tubulin promoter (18). The k values (mean ± SD) are calculated from the proportion of the total offspring (n) that received the Rspi cn+ chromosome. The SD components present in each of the tested genotypes are shown in brackets. See Materials and Methods for details of fly crosses and chromosome markers.