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. 2002 May 14;99(10):6871–6876. doi: 10.1073/pnas.072290599

Figure 2.

Figure 2

Caspase 2 interacts with cyclin D3 in mammalian fibroblasts by transfection and immunoprecipitation (IP) Western analysis. IP Western showing that cyclin D3 interacts with Caspase 2 in HEK 293 cells and not with Caspase 2-Myc. (Top) HEK 293 cells transfected with the indicated plasmids. + indicates the plasmid labeled at the right; − indicates the corresponding vector control. (Middle) Cell lysates immunoprecipitated with anti-Myc and immunoblotted with anti-HA. HA-cyclin D3 is detected at 33 kDa. (Bottom) Expression of the corresponding proteins. For Caspase 2-Myc, we immunoprecipitated cell lysates with anti-Myc antibody and probed a blot of the resulting gel with anti-Myc antibody. For Caspase 3-Myc and HA-cyclin D3, we probed the blot with anti-Myc and anti-HA antibody, respectively. Panel shows that cyclin D3 coprecipitates with Caspase 2-Myc, but not with the Myc-tagged vector control, and not with Caspase 3-Myc.