Table 2.

Directed synthesis of cyclin D3 and Caspase 2 results in increased cell death compared with directed synthesis of Caspase 2 alone

Transfection	Percent death (±SD)
Cyclin D3/Caspase 2	59 (±13)
Caspase 2	38 (±6)
Cyclin D3	5 (±2)
Control	4 (±2)

We transfected HEK 293 cells with constructs directing the expression of cyclin D3 (pACL4-cyclinD3) and Caspase 2 (pcDNA3-caspase-2), Caspase 2 (pcDNA3-caspase-2), and a vector control (pACL-4), cyclin D3 (pACL4-cyclinD3) and a vector control (pcDNA3.1), or two expression vector controls (pcDNA3.1 and pACL4). Percent death is the fraction of GFP-expressing cells that appear dead under phase microscopy in two independent experiments. Green cells correspond to those expressing GFP and either cyclin D3 for pACL4–CyclinD3 or a noncoding insert for control vector pACL-4.