Figure 3.

AGO1 mutant embryos have a reduced response to eve dsRNA. Representative embryonic phenotypes of wild-type, GFP-expressing AGO1⁺, and AGO1^k08121 mutant embryos injected with dsRNA are shown. (A) w¹¹¹⁸ embryo injected with white dsRNA showing no alteration in the number of ventral denticle belts. (B) w¹¹¹⁸ embryo injected with eve dsRNA. Denticle belt 4 is fused with 5, and 6 is missing. (C) AGO1⁺ embryo injected with eve dsRNA. Belt 6 is missing. (D) AGO1^k08121 embryo injected with eve dsRNA showing no alteration in the number of denticle belts. (E) AGO1⁺ embryo injected with eve siRNA. Belt 4 is partially formed whereas belt 6 is missing. (F) AGO1^k08121 embryo injected with eve siRNA showing no alteration in the number of denticle belts. The denticle belts corresponding to each abdominal segment are labeled, except 8, which is not in the plane of focus.