Table 1.
mAb | Epitope | Env | Competition
|
|
---|---|---|---|---|
−sCD4 | +sCD4 | |||
17b | CD4i | 89.6 | + | ++ |
17b | CD4i | JR-FL | + | ++ |
CG10 | CD4i | 89.6 | 0 | ++ |
CG10 | CD4i | JR-FL | 0 | +++ |
A32 | CD4i(C1–C4) | 89.6 | −−− | − |
A32 | CD4i(C1–C4) | JR-FL | −− | −− |
48d | CD4i | 89.6 | + | + |
23e | CD4i | 89.6 | + | + |
21c | CD4i | 89.6 | + | + |
F91 | CD4bs | JR-FL | + | NA |
IgG1b12 | CD4bs | JR-FL | + | NA |
19b | V3 | JR-FL | 0 | 0 |
G3-136 | V2 | JR-FL | 0 | 0 |
MAG45 | C1 | JR-FL | 0 | 0 |
C11 | C1–C5 | JR-FL | 0 | 0 |
2G12 | C3/V4 | JR-FL | 0 | 0 |
G3–519 | C4 | JR-FL | 0 | 0 |
X5 | CD4i | 89.6 | +++ | +++ |
Gp120JR-FL was captured by the anti-gp120 Ab D7324 and incubated with X5 (5 μg/ml) and various antibodies at different concentrations in absence or presence of sCD4 (2 μg/ml), and IgG Fc of the bound antibodies was detected with goat anti-mouse or anti-human IgG Fc alkaline phosphatase conjugate. Gp12089.6 was directly coated on 96-well plates and incubated with biotinylated X5 (0.5 μg/ml) and various antibodies, and the amount of bound X5 was detected by using streptavidin-HRP. +, Denotes some competition; ++, significant competition; +++, complete displacement; −, various degrees of enhancement; 0, lack of measurable effects; NA, not applicable.