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ATP6S1 is immunogenic in murine melanoma. Sera from C57BL/6 mice vaccinated with irradiated GM-CSF-secreting B16 cells or from naive controls were evaluated by Western analysis (1:300 dilution) against GST-ATP6S1 (human) and GST-BRAP2 (human) (anti-mouse IgG secondary antibody). Murine ATP6S1 shows 90% and murine BRAP2 shows 95% sequence similarity to the respective human homologs. (A) Naive. (B) Vaccinated.
