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. 2002 May 14;99(10):6937–6942. doi: 10.1073/pnas.102645699

Figure 1.

Figure 1

Generation of mice overexpressing different peptide–Ab complexes. (A) Schematic of the human invariant chain locus. The Eα, CD22, and Rab peptide sequences replacing CLIP in each of the Ii-peptide Tgs are shown. (B) Splenocytes from C57BL/6, H-2M0, and each of the Ii-peptide mice were stained with antibodies binding Ab (Y3P), mouse CLIP–Ab (15G4), Eα–Ab (YAe), CD22–Ab (A8), and Rab–Ab (H10). Staining of peptide-Ii0 mice are indicated by thin, black lines; peptide-dbl0 mice are indicated by thick, gray lines. (C) Stimulation of T cell hybridomas specific for CD22 (25–39)–Ab, IgM (377–392)–Ab, β2m (48–58)–Ab, or Rab (86–101)–Ab by titrated splenocytes from the indicated mice is shown. IL-2 production was measured by using the IL-2-dependent cell line HT-2. The data are presented as a mean OD570/600 of duplicate cultures.