Figure 4.

CD4 T cells from Ii-peptide mice have different specificities. Proliferative responses of CD4 T cells from Eα-dbl⁰, CD22-dbl⁰, H-2M⁰, and C57BL/6 mice immunized with OVA265-277 peptide are shown. CD4 T cells isolated from Eα-dbl⁰, CD22-dbl⁰, Rab-dbl⁰, and H-2M⁰ mice (B), from reciprocal BM chimeras between Eα-dbl⁰ and H-2M⁰ mice (C), or from Eα-Ii⁰, CD22-Ii⁰, and Rab-Ii⁰ mice (D) were stimulated with irradiated syngeneic (open bars) or C57BL/6 (closed bars) splenocytes. The data are presented as the mean [³H]thymidine incorporation of triplicate cultures. In the experiments shown in A, the SEM was always less than 20% of the mean cpm of triplicate cultures. WT, wild type.